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USA 99— Parasite load, body size, and age of wild-caught male field crickets Orthoptera: Acknowledgements We thank C. Physical contact is probably important [ 45 ], but the use of tactile cues is limited to situations in which population density is already high enough to permit frequent encounters between individuals. Because of their highly conspicuous nature, long-range sexual signals are prime candidates to be used in evaluating the social conditions likely to be experienced upon adulthood in continuously breeding species; however, their role in mediating immune responses is unknown. Phenoloxidase is stored in haemolymph as an inactive precursor, pro-phenoloxidase.

The phenoloxidase assay spectrophotometrically measures haemolymph levels of phenoloxidase, an enzyme necessary for melanin production and hence important in invertebrate immunity.

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Evolution 42— Phenoloxidase is stored in haemolymph as an inactive precursor, pro-phenoloxidase. Adult males exposed to song during rearing showed stronger immune responses than males reared in silence: To test whether exposure to acoustic signals stimulates predictable increases in immune investment, and whether the two male morphs show intrinsic differences in immune investment, we manipulated male acoustic experience during rearing using playbacks. Phenoloxidase converts l -DOPA to dopachrome, causing the solution to darken.








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